megalign module (DNASTAR)
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DNASTAR
megalign module
Megalign Module, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 5885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/megalign+module/MegAlign/pm41932427-104-7-10
Average 99 stars, based on 5885 article reviews
Megalign Module, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 5885 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/megalign+module/MegAlign/pm41932427-104-7-10
Average 99 stars, based on 5885 article reviews
megalign module - by Bioz Stars,
2026-10
99/100 stars
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Sequencing:Article Title: Comprehensive study of tropical theileriosis in Assiut province, Egypt, with reference to molecular findings in diseased cattle Article Snippet: This study investigated the infection rate among clinically suspected cases, clinical aspects, pathological lesions and molecular characteristics of Theileria annulata in clinically suspected cases of cattle and buffaloes from Assiut Governorate, Upper Egypt.. A total of 600 animals (500 cattle, 100 buffaloes) were examined using microscopy and PCR, revealing an infection rate of 32.2% in cattle and 20% in buffaloes.. Clinical examination showed common signs of theileriosis, with infection rate significantly higher during the hot season. Article Title: Marek's disease virus UL16 tegument protein is essential for secondary envelopment and infectious virion formation. Article Snippet: Marek’s disease virus (MDV) is a highly oncogenic alphaherpesvirus whose productive infection and cell-to-cell spread rely on the formation of infectious mature virions, a process mediated by tegument proteins.. UL16 is a conserved herpesviral tegument protein, in other herpesviruses, its deletion causes severe replication defects.. However, its role in MDV remains unknown. Article Title: Isolation and characterization of a fowl adenovirus 2 (FAdV-2) strain YN2497 associated with lethal hydropericardium-hepatitis syndrome in neonatal chickens in China. Article Snippet: Since 2015, the detection rate of fowl adenovirus (FAdVs), including FAdV-2, has increased steadily in China, posing growing challenges to the prevention and control of avian diseases.. In this study, an FAdV strain, designated YN2497, was isolated from chickens in Yunnan Province, China, and its genomic characteristics and pathogenicity were investigated.. Whole-genome sequencing and comparative phylogenetic analysis confirmed that strain YN2497 belongs to serotype FAdV-2 within the species Fowl Aviadenovirus D. Experimental infection of 1-day-old specific-pathogen-free (SPF) chicks induced typical hydropericardium-hepatitis syndrome (HHS), with viral shedding detected in both oral and cloacal swabs starting at 1-day post-infection. Article Title: Viral isolation and genomic characteristics of the first bovine parainfluenza virus type 3 isolated from water buffaloes (Bubalus bubalis) in China. Article Snippet: Bovine parainfluenza virus type 3 (BPIV3) is an important pathogen associated with bovine respiratory disease.. In this study, we report the isolation and genomic characterization of BPIV3 from a water buffalo with respiratory symptoms in China.. Virus isolation was performed using susceptible cell cultures, followed by identification via RT-qPCR, transmission electron microscopy, and indirect immunofluorescence. Article Title: Epidemiological Study and Molecular Characterization of Lumpy Skin Disease in Cattle in Egypt Article Snippet: A purified PCR product of three field samples (skin nodules) was collected from 3 cows (1st cow during June 2025 after vaccination with the AL‐Abassya LSD vaccine, 2nd cow during September 2025 after vaccination with an unknown type of LSD vaccine, and 3rd cow during September 2025; it is not known if it was vaccinated or not) and the Neethling strain of ARRIAH LSD VAC (The trade name for ARRIAH LSD vaccine, and it is commercial Russian vaccine, was recently introduced in Egypt in 2025 and manufactured by the Federal Center for Animal Health (FGBI′′ARRIAH′′)), was sequenced in one direction on an Applied Biosystems 3130 automated DNA sequencer (ABI, 3130, USA), utilizing a BigDye Terminator V3.1 cycle sequencing kit (Cat. No. 4336817) with a ready reaction (Perkin‐Elmer/Applied Biosystems, Foster City, CA). .. The CLUSTAL W multiple sequence alignment approach, created by Thompson et al. [ ] and accessible in Version 1.83 of the Software:Article Title: Comprehensive study of tropical theileriosis in Assiut province, Egypt, with reference to molecular findings in diseased cattle Article Snippet: This study investigated the infection rate among clinically suspected cases, clinical aspects, pathological lesions and molecular characteristics of Theileria annulata in clinically suspected cases of cattle and buffaloes from Assiut Governorate, Upper Egypt.. A total of 600 animals (500 cattle, 100 buffaloes) were examined using microscopy and PCR, revealing an infection rate of 32.2% in cattle and 20% in buffaloes.. Clinical examination showed common signs of theileriosis, with infection rate significantly higher during the hot season. Article Title: Marek's disease virus UL16 tegument protein is essential for secondary envelopment and infectious virion formation. Article Snippet: Marek’s disease virus (MDV) is a highly oncogenic alphaherpesvirus whose productive infection and cell-to-cell spread rely on the formation of infectious mature virions, a process mediated by tegument proteins.. UL16 is a conserved herpesviral tegument protein, in other herpesviruses, its deletion causes severe replication defects.. However, its role in MDV remains unknown. Article Title: Molecular detection and sequencing of the NetB toxin Gene of clostridium perfringens and evaluation of its pathogenicity in broiler chicken Article Snippet: .. The obtained NetB gene sequences were edited and aligned using the CLUSTAL W algorithm integrated in the Article Title: Molecular epidemiology and genetic evolution of PRRSV ORF5 in Sichuan, Southwest China Article Snippet: .. To characterize genetic variability in the encoded GP5 protein, the aligned amino acid sequences were further analyzed and visualized using the Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons. Recombinase Polymerase Amplification:Article Title: Rapid detection of Streptococcus agalactiae in tilapia using RPA-PfAgo detection systems Article Snippet: Streptococcus agalactiae (GBS), a leading tilapia pathogen, causes high mortality with frequent asymptomatic infections, complicating early detection.. The study focused on GBS and employed recombinase polymerase amplification (RPA) technology in conjunction with Pyrococcus furiosus Argonaute (PfAgo) to establish real-time fluorescent RPA-PfAgo (RPA-PfAgo-RTF) and lateral flow strip-based RPA-PfAgo assay (RPA-PfAgo-LFD).. The conserved cfb gene was selected for the design and screening of primers, gDNAs, and molecular beacons. |